Page 259 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PP-76

Antcin K inhibits cell migration in osteosarcoma cells

2
1
3
Chih-Hsin Tang, Yueh-Hsiung Kuo, Ming-Lung Hsu, Jing-Sheng Chien, Meng-Yu
3
Liang, Po-Chun Chen*
,3
3

1 Department of Pharmacology, China Medical University School of Medicine
2 Department of Chinese Pharmaceutical Sciences and Chinese Medicine, China Medical
University School of Medicine
3 School of life science, National Taiwan Normal University
* E-mail: pcchen@ntnu.edu.tw

Abstract
Osteosarcoma is a malignant tumor that grows in the bone and is common in teenagers and
adults. Without metastasis, the survival rate within five years can reach 60-70%, but if it occurs,
the survival rate will be less than 20%. Metastasis refers to the movement of cancer cells from
the primary organ to other organs through the bloodstream. This process involves the
weakening of intercellular adhesion and the degradation of extracellular matrix, and the
adaptability of cancer cells to the new environment is also a factor to determine the success of
metastasis. The metastasis of osteosarcoma most frequently occurs and affects the liver,
resulting in a decline in treatment efficacy and poor prognosis for patients. At present, we do
not have a full understanding of the metastasis mechanism of osteosarcoma and the treatment
of it in terms of anti-metastasis. Therefore, it is very important to develope new treatment for
the metastasis of osteosarcoma cells. A medicinal fungus, Antrodia camphorata, contains a
triterpene compound, Antcin K. Current studies have shown that it has anti-tumor and
immunomodulatory functions. In the research of hepatocellular carcinoma, Antcin K has been
proven to induce apoptosis, inhibit the migration and invasion of cancer cells. However, no
research has investigated the anti-cancer effect of Anticin K on osteosarcoma. Our preliminary
research indicates that Antcin K does not affect the viability of two osteFosarcoma cell lines
(HOS and 143B) at a high concentration of 40 μM. But through metastasis experiments, we
tested three concentrations (20 μM, 40 μM, and 80 μM) of Antcin K, which could significantly
inhibit two osteosarcoma cell lines (HOS and 143B) and showed significant effects at the 80
μM concentration.

Keywords: Osteosarcoma; Cancer; Antrodia camphorata; Antcin K