Page 244 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PP-61

SWITCHER: A novel tool for precise ev subpopulation isolation and

nondestructive release with the SHINER workflow

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1
1
Jhih-Fong Li, Chen-Wei Hsu, Yao-Ching Fang, Chi-An Cheng*
#,1

1 School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan
* E-mail: cacheng123@ntu.edu.tw

Abstract
Extracellular vesicles (EVs) have gained attention in disease diagnosis and therapeutic
applications due to their diverse roles in cellular communication. However, their natural
variability poses a challenge, limiting their full potential. Traditional EV isolation methods
typically rely on biophysical characteristics, making it difficult to efficiently separate EV
subpopulations based on molecular markers. Even affinity-based methods, which can target
specific EV subtypes, often involve harsh recovery steps that can damage the EVs. To overcome
these challenges, we present SHINER, a new approach to EV isolation and recovery. Central to
SHINER is the innovative SWITCHER tool, which works in combination with the
ultrasensitive eSimoa platform we previously developed. SHINER achieves two key objectives:
(1) the precise separation and gentle retrieval of specific EV subpopulations from complex
biological samples, and (2) the preservation of the structural integrity and biological
functionality of the recovered EVs. SHINER enhances the ability to trace the origins of EVs
for diagnostic purposes, opens new avenues in EV research, and provides standardized EVs for
therapeutic applications. This breakthrough not only advances our understanding of EV biology
but also paves the way for personalized diagnostic and treatment strategies, pushing the field
of EV research to new heights.

Keywords: Extracellular Vesicles (EVs); Affinity-Based Isolation; CD81-Rich EVs; Single
Molecule Array (eSimoa)