Page 248 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PP-65

Effects of acetylated glucomannan derived from Dendrobium Taiseed

Tosnobile on non-alcoholic fatty liver disease and gut microbiota modulation

in mice

,3
1,2
Chun-Hui Chiu, Li-Chan Yang*

1 Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung
University of Science and Technology, Taoyuan 333, Taiwan
2 Department of Nephrology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
3 Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing
University, Taichung 40227, Taiwan
* E-mail: lcyang@dargon.nchu.edu.tw

Abstract
Non-alcoholic fatty liver disease (NAFLD) is a prevalent chronic liver condition worldwide,
often progressing to non-alcoholic steatohepatitis (NASH), fibrosis, and even hepatocellular
carcinoma. Current treatments are limited, with a growing need for alternative therapeutic
options. This study investigated the effects of acetylated glucomannan polysaccharides derived
from a cultivated hybrid medicinal Dendrobium, Dendrobium Taiseed Tosnobile (DTTPS), on
NAFLD using a high-fat, high-cholesterol diet-induced mouse model. The chemical structure
and acetylation degree of DTTPS and its deacetylated form (deac-DTTPS) were characterized.
Mice were orally administered with DTTPS or deac-DTTPS for 16 weeks alongside high-fat
feeding. Results demonstrated that DTTPS significantly ameliorated liver steatohepatitis,
reduced serum levels of glucose, triglycerides, cholesterol, ALT, and AST, and decreased
hepatic lipid accumulation. These effects were accompanied by improved hepatic antioxidant
status, including increased glutathione (GSH) content. Deacetylated polysaccharides exhibited
moderately diminished efficacy, highlighting the critical role of acetyl substituents in
bioactivity. Importantly, DTTPS modulated gut microbiota composition by increasing the
abundance of the beneficial bacterium Akkermansia muciniphila and elevated urinary levels of
microbial metabolites indole-3-acetic acid and 4-hydroxyphenylacetic acid, which are known
to have anti-inflammatory and antioxidative properties. These factors likely contributed to the
observed liver protection through the gut-liver axis. In conclusion, acetylated glucomannan
from Dendrobium Taiseed Tosnobile demonstrates promising hepatoprotective effects against
diet-induced NAFLD by modulating gut microbiota and enhancing antioxidant defense. This
study supports the potential development of DTTPS as a functional herbal ingredient for
improving metabolic liver diseases and highlights the importance of acetylation in
polysaccharide bioactivity.

Keywords: Dendrobium Taiseed Tosnobile; Glucomannan; Acetyl group; Non-alcoholic fatty
liver disease; Gut microbiota