Page 266 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PP-83

Evaluation of the anti-metastatic effects of Taiwan Cryptomeria japonica

extract on colorectal cancer

2
,4
1
Y u -Hsiang Hung, 1,2,3,4 Shang Tse Ho, Yi-Wen Liu, Chi-Chien Lin*

1 Graduate Department of Wood Based Materials and Design, National Chiayi University,
Chiayi 60077, Taiwan
2 Distinguished Professor at Department of Microbiology, Immunology and
Biopharmaceuticals, National Chiayi University, Chiayi 60077, Taiwan.
3 Institute of Biomedical Sciences, National Chung Hsing University, Taichung 402201,
Taiwan
4 Master Program in Precision Health, Taichung 402201, Taiwan
* E-mail: chichenlin1@gmail.com

Abstract
The heartwood of Cryptomeria japonica, an endemic species in Taiwan, is rich in diverse
bioactive metabolites, including phenolic diterpenes, oxidized diterpenes, phytosterols,
flavonoids, lignans, and sesquiterpenes. Although previous studies have demonstrated its
antioxidant, anti-inflammatory, antibacterial, and insecticidal properties, its anticancer potential
has not been clearly elucidated. The present study aimed to investigate the cytotoxic effects of
C. japonica heartwood extracts on colorectal cancer cells, with a particular focus on cellular
senescence and cell cycle regulatory mechanisms. Furthermore, validation using a C57BL/6
mouse model bearing metastatic tumors revealed that treatment with the extracts exerted anti-
metastatic effects in a dose-dependent manner. This study provides the first experimental
evidence supporting the anticancer activity of C. japonica heartwood, highlighting its potential
as a natural adjuvant resource for cancer prevention and therapy.

Keywords: Cryptomeria japonica; Colorectal cancer; Anti-metastatic; Cellular senescence;
Cell cycle