Page 271 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

CM-04

Main bioactive components and their biological activities from the root of

Scutellaria baicalensis

1
Wen-Hsin Hu (胡文馨), Jih-Jung Chen (陳日榮)* ,1

1 Department of Pharmacy, College of Pharmaceutical Sciences, National Yang Ming Chiao
Tung University, Taipei 112304, Taiwan-
* E-mail: jjungchen@nycu.edu.tw

Abstract
Scutellaria baicalensis, a traditional Chinese medicinal herb, was investigated for its
antioxidant, anti-inflammatory, and anti-ultraviolet activities in various solvent extracts and
isolated compounds in this study. The ethyl acetate extract exhibited strong antioxidant activity
in the DPPH assay (SC50 = 14.80 μg/mL), the acetone extract showed excellent antioxidant
activity in both ABTS (SC50 = 9.04 μg/mL) and FRAP assays (TE = 1526.37 mM/g), whereas
the 100 °C water extract demonstrated the highest antioxidant activity in the superoxide assay
(SC50 = 34.89 μg/mL). Most extracts outperformed the positive controls, BHT (SC50 = 118.11
and 7.33 μg/mL on DPPH and ABTS, TE = 3450.33 mM/g on FRAP). The dichloromethane
extract exhibited potent anti-inflammatory activity (IC50 = 21.01 μg/mL) in RAW264.7 cells,
whereas the 100 °C water extract demonstrated anti-ultraviolet and antioxidant effects in
HaCaT cells. Eight major compounds were isolated. Among them, scutellarein (6) exhibited
stronger antioxidant activity (SC50 = 13.70 μM on DPPH, 12.42 μM on ABTS) than BHT (SC50
= 227.77 μM on DPPH, 42.32 μM on ABTS). Baicalein (2) (TE = 1216.94 μM) exhibited
significantly greater FRAP activity than BHT (SC50 = 890.88 μM). Scutellarin (5) displayed the
highest antioxidant activity in the superoxide assay (SC50 = 34.74 μM), approaching that of
cynaroside (SC50 = 28.55 μM). Furthermore, wogonoside (3) not only inhibited NO production
(IC50 = 10.18 μM) in RAW264.7 cells but also protected HaCaT cells from ultraviolet-induced
damage. This research suggests that bioactive extracts and components from S. baicalensis
could be hopeful candidates for the prevention or treatment of oxidative stress, inflammation,
and ultraviolet-induced damage.

Keywords: Scutellaria baicalensis; Anti-inflammatory; Antioxidant; Anti-ultraviolet
radiation