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CM-05

Evaluation of aroma omposition and anti-inflammatory activity of MOA-EO,

a mixed essential oil derived from Magnoliae Flos, Osmanthus Flowers, and

Agarwood

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Yi-Jen Wang, Adeel Akram, Wen-Te Chang, Ming-Kuem Lin*

1 Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China
Medical University, Taichung, Taiwan
* E-mail: linmk@mail.cmu.edu.tw

Abstract
Traditional Chinese Medicine has long been recognized as a prolific source of natural
bioactive agents, characterized by multi-component, multi-target, and synergistic effects,
particularly in immune regulation and inflammation suppression. Magnoliae Flos (M),
Osmanthus Flowers (O), and Agarwood (A) have individually been reported to possess
immunosuppressive, antioxidant, and anti-inflammatory properties. To develop a cosmetic
product based on aromatic anti-inflammatory essential oils, a blend termed MOA-EO was
formulated by combining these three essential oils. Both fragrance acceptability and
macrophage-mediated immune modulation were evaluated. Six MOA-EO formulations
(Groups A–F), prepared with varying ratios of the three oils, were subjected to sensory
evaluation by 40 participants to assess fragrance preference. The top three formulations (Groups
A, D, and F) were subsequently examined in vitro. Using RAW 264.7 murine macrophages as
the cellular model, MTT assays were conducted across five concentration gradients to evaluate
cell viability. Group A, D and F exhibited no cytotoxicity at concentrations 1 (M: 70%, O: 15%,
A: 0.5%) and 2 (M: 35%, O: 7.5%, A: 0.25%). These two concentrations were therefore selected
for further investigation of anti-inflammatory effects and underlying mechanisms. This study
aims to identify the most favorable fragrance with anti-inflammatory activity among the MOA-
EO formulations, providing a foundation for the development of cosmetic and other anti-
inflammatory products.

Keywords: Anti-inflammatory activity; Cytotoxicity; Fragrance evaluation; Cosmetic
applications