Page 324 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

TC-09

Chemical constituents and hepatoprotective effects of Helicteres hirsuta

water extract

1
,1
1
Thi Van Anh Tran,* Thi Hong Tuoi Do, Van Ho Nam Phan, Thi Kim Anh Le, Phuong
2
2
Ngoc Anh Le, Phan Thao Trang Nguyen
1

1 School of Pharmacy, University of Medicine and Pharmacy at HoChiMinh city, HoChiMinh
city 700000, Vietnam
2 Saigon Pharmaceutical Science and Technology Center, 70000, Vietnam
* E-mail: ttvananh@ump.edu.vn

Abstract
Helicteres hirsuta has been utilized in Vietnamese traditional medicine for the treatment of
liver diseases. Despite numerous investigations on this plant, there is a deficiency of knowledge
on the chemical properties and pharmacological effects of the aqueous extract of H. hirsuta,
which is the traditional usage. This study compared the hepatoprotective activities and
antioxidant properties of ethanol and water extracts of H. hirsuta in in vitro models, revealing
that the water extract (HEW) demonstrated significant efficacy. Phytochemical study was
conducted on HEW to identify danshensu, rosmarinic acid, and tiliroside as the principal
compounds, along with five flavonoids and two phenolic compounds that were isolated from
H. hirsuta for the first time. An HPLC method for the simultaneous quantification of three
markers of HWE was established for the quality control of the extract utilized in the in vivo test
to evaluate its hepatoprotective properties. The HWE at doses of 412.5 mg/kg and 825 mg/kg
has hepatoprotective properties against paracetamol-induced liver injury in mice. The HWE
contributed to lowering MDA levels, enhancing GSH, diminishing liver enzymes (AST, ALT
and ALP), and total bilirubin, as well as reducing both macroscopic and microscopic liver
damage. It is concluded that HEW demonstrates hepatoprotective effects similar to those
observed in traditional medicine. The effects may be elucidated by the properties of identified
constituents in HWE. The results of this study could be the scientific evidence for developing
the healthcare product of H. hirsuta in the future.

Keywords: Helicteres hirsuta; Hepatoprotective; Danshenhu; Rosmarinic acid; Tiliroside