Page 123 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PC-06

Primary and secondary metabolite profiles of the solid-state cultured

Armillaria sp. (TAS) from ABF germplasm bank

2
1
#,1
3
Chih-Li Chen, Chao-Yu Chen, Hsien-Hung Chang, Mohamed El-Shazly, Ming-
1,5
2
1
4
1
Chung Lee, Y u -Ming Hsu, Ying-Chi Du, Y u -Hsuan Lin, Shih-Tsun Liang, Tun-Tschu
Chang, Chuen-Hsu Fu*
1
,1

1 Taiwan Forestry Research Institute, Ministry of Agriculture, Taipei 100051, Taiwan
2 Herbiotek Co., Ltd., New Taipei City 231030, Taiwan
3 Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Organization
of African Unity Street, Abassia, Cairo 11566, Egypt
4 Research Center for Precision Environmental Medicine, Kaohsiung Medical University,
Kaohsiung City 807378, Taiwan
5 Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944401,
Taiwan
* E-mail: fch@tfri.gov.tw

Abstract
Armillaria species, commonly known as honey mushrooms, are valued in Europe and Asia
for their medicinal and culinary applications. This genus of Basidiomycota fungi is recognized
for its bioactive secondary metabolites, which exhibit antioxidant, anti-inflammatory, anti-
aging, anti-diabetic, anti-tumor, hypolipidemic, immunoregulatory, and hepato-pulmonary
protective properties. Over 40 polysaccharide types have been isolated from Armillaria mellea
fruiting bodies, hyphae, and fermentation broth, underscoring its phytochemical diversity.
Established in 2021 by Dr. Tun-Tschu Chang and Dr. Chuen-Hsu Fu, the Applied Biomedical
Fungal Germplasm Bank (ABF Germplasm Bank) houses over 500 fungal samples, including
Antrodia, Armillaria, Lignosus, Phellinus, and Trametes genera, focusing on biomass
evaluation and pharmacochemical research. This study investigates the metabolite profile of
Armillaria sp. (TAS), a strain from Taiwan cultured via solid-state fermentation. We analyzed
primary metabolites (carbohydrates, proteins, lipids) with Food Testing Methods and secondary
metabolites (phenolics, polysaccharides, diterpenoids, triterpenoids, alkaloids, sterols) using
1
extraction, liquid-liquid partitioning, RP-HPLC-UV, and H-NMR techniques. Results revealed
that the polysaccharide-rich aqueous layer was the most abundant fraction, highlighting TAS’s
potential as a functional food and medicinal resource.

Keywords: Armillaria sp.; Fungal germplasm bank; Solid-state culture; Metabolite profile