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CM-35

Effects of the Traditional Chinese Medicine formula Zhu Pi Da Wan on

offspring gut microbiome and intestinal transcriptome in a postpartum

mouse model

2,3
1
Chung-Chih Liao, De-Wei Lai, Jung-Miao Li,* ,4,5

1 Department of Integrated Chinese and Western Medicine, Chung Shan Medical University
Hospital, Taichung, Taiwan
2 Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan
3 Experimental Animal Center, Department of Molecular Biology and Cell Research, Chang
Bing Show Chwan Memorial Hospital, Changhua, Taiwan.
4 School of Chinese Medicine, College of Chinese Medicine, China Medical University,
Taichung, Taiwan
5 Department of Chinese Medicine, China Medical University Hospital, Taichung, Taiwan
* E-mail: jmli@mail.cmu.edu.tw

Abstract
Traditional Chinese Medicine (TCM) is widely used for postpartum recovery. Zhu Pi Da Wan,
a classic formula from the Jin Gui Yao Lue traditionally used for postpartum care, has yet to
have its underlying mechanisms fully elucidated. This study investigates its influence on the
gut microbiome and intestinal immune development in offspring through maternal
administration. Postpartum C57/BL6 dams were orally administered either Zhu Pi Da Wan or
a vehicle control for three weeks. The gut microbiome of the pups was analyzed via 16S rRNA
gene sequencing of fecal samples, and their intestinal tissue was subjected to RNA sequencing
(RNA-Seq) to evaluate transcriptomic changes. Maternal supplementation with Zhu Pi Da Wan
significantly altered the gut microbial community structure in the pups. Notably, the treatment
group showed a significant enrichment of beneficial bacteria, including Akkermansia, and a
lower Firmicutes/Bacteroidota ratio compared to the control group. Transcriptomic analysis of
the pup intestines revealed a distinct gene expression profile. Differentially expressed genes
were significantly enriched in immune-related pathways, including the B cell receptor signaling
pathway, Th17 cell differentiation, and the intestinal immune network for IgA production, as
well as pathways associated with fat digestion and absorption. Our findings demonstrate that
maternal administration of Zhu Pi Da Wan during the postpartum period profoundly modulates
the offspring's gut microbiota and intestinal transcriptome. The formula promotes a healthier
microbial profile and appears to prime the intestinal immune system, offering a potential
mechanistic explanation for its traditional therapeutic benefits in postpartum maternal and
infant health.

Keywords: Zhu Pi Da Wan; Postpartum; Gut microbiome; RNA-seq; Intestinal immunity