Page 332 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

TC-17

A novel botanical supplement improves physical performance and immune

senescence via regulating systemic metabolism and muscle-gut-axis in aged

mice

1
1
1
2
1
Meng-Ting Chang, Chi-Chang Huang, Y u -Ling Lin, Hui-Ming Chen, Tai-Na Wu,
Lie-Fen Shyur *
,1

1 Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan
2 Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 333,
Taiwan
* E-mail: jaclyn@gate.sinica.edu.tw

Abstract
According to the World Health Organization, by 2030, one in six people globally—
approximately 2.1 billion individuals—will be over the age of 60. Taiwan has already become
an super aged society in 2025 with more than 20% of its population aged 65 and older. This
study intends to explore functional botanical ingredients which exhibit anti-aging effects. We
identify a active pharmaceutical ingredients from an Asteraceae medicinal plant (namely CAI)
that enhances physical performance, muscle strength and endurance in aged mice. CAI prevents
TNFα-induced muscle atrophy in C2C12 myoblast cells. CAI shows significant
immunomodulatory effects, reduces inflammaging and aging-associated B cell (ABC)
population in aged mice. LC-MS-based metabolomics and metabolic pathway analyses of
mouse sera or muscles revealed that CAI supplementation significantly modulates the
metabolism of aromatic amino acids in aged mice. Furthermore, CAI modulates bioactive lipid
mediators, namely oxylipins profiles and dynamics and targeting specific metabolic pathways
that may associate to muscle and immune functions in aged mice. Several mitochondria and
energy metabolism associated genes were upregulated by CAI supplementation in aged mouse
muscles. Gut microbiome analysis using next generation gene sequencing revealed that CAI
reduces pathogenic bacteria and increases beneficial probiotics abundances. Of note, CAI
modulates gut bacterial population exhibiting correlation with both primary and lipid
metabolites, suggesting that CAI regulates systemic and muscle-gut-axis toward preventing
immune aging and muscle loss in aged mice that might be useful in combating sarcopenia in
older adults.

Keywords: Aging; Botanical supplement; Sarcopenia; Immune senescence; Metabolism and
gut microbiota