Page 334 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

TC-19

Phenotype characterization to estimating the efficacy of monocytic cell-

based immunotherapy after modulation by traditional Chinese medicine

,1
1
1,2
Hsin-Ju Y u , Y u -Hsiang Hsu, Chen-Fang Lin, Ren-In You*
1

1 Department of Laboratory Medicine and Biotechnology, Tzu Chi University, College of
Medicine, Hualien, Taiwan.
2 Division of General Surgery, Department of Surgery, Hualien Tzu Chi Hospital, Buddhist
Tzu Chi Medical Foundation, Hualien, Taiwan.
* E-mail: yri100@gms.tcu.edu.tw

Abstract
Traditional Chinese medicine targeting the immune network and combining various targeted
therapy strategies has promoted the development of personalized medicine, providing a variety
of collocation options in the treatment of chronic diseases, such as cancer or autoimmunity.
Huang Lian Chieh Dwu are considered as potent immunomodulatory properities such as anti-
inflammatory, antioxidant and against inflammatory cytokine storm. To simplify the evaluation
of immunomodulatory effect of blood cell products, the indicators that provided efficacy
monitoring are worth developing and interpreting. Monocytes from peripheral blood
mononuclear cells (PBMC) were isolated in vitro using magnetic beads negative selection with
autoMAC for preparing, tumor lysates or self-antigen peptides were incubated with various
matrixes and further co-culture with T cells. The degree of T cell activation was examined using
antigen-loaded MHC tetramer and T cell polarization was analyzed by intracellular staining.
The level of cell survival and proliferation were evaluated by automated cell counters, PI and
CFSE staining. We found that the appropriate ratio of monocytes to T cells affects the activation
strength of T cell polarization. In addition, monocytes are stimulated by higher concentrations
of tetracyclic isoquinoline compounds from Huang Lian Chieh Dwu, which modulate their
attractiveness to T cell and affect T cell polarization. Thus, dose-relationships combined Huang
Lian Chieh Dwu with anti-cancer or anti-inflammatory drugs can indicate the type of immune
response that is inflamed or suppressed. Efficacy of monocyte immunotherapies categorized by
these parameters will facilitate easy screening and development of personalized treatments in
anticipation of responsiveness or quality.

Keywords: Huang Lian Chieh Dwu; Monocyte; Immunomodulatory