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PP-09

Metabolomics-based evaluation of Allium hookerii Thwaites in non-insulin-

dependent diabetes mellitus and dyslipidaemia

1
,1
Barun Das Gupta,* Pallab Kanti Haldar, Pulok Kumar Mukherjee 1

1 School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur
University, Kolkata – 700032, West Bengal, India
* Email: barundasgupta38@gmail.com

Abstract
Allium hookerii Thwaites belonging to the family Liliaceae, commonly known as hooker
chive is traditionally used to manage blood sugars and hypertension. This study aims the
prophylactic intervention of non-insulin-dependent diabetic mellitus and dyslipidaemia using
the hydroalcoholic extract of A. hookerii and investigate the mechanism utilising
metabolomics-based network pharmacology. A validated microwave-assisted extraction
method produced A. hookerii hydroalcoholic extract. The in-vitro α-glucosidase, α-amylase,
and pancreatic lipase inhibition test assessed anti-hyperglycaemic and anti-hyperlipidaemic
capabilities. The hydroalcoholic extract was tested in high-fat and high-sugar fed C57BL/6J
mice for anti-hyperglycaemic and anti-hyperlipidaemic activities using metformin and
atorvastatin as reference standards. Metabolomics integrated network pharmacology study
revealed the mechanism. A. hookerii hydroalcoholic extract inhibited α-glucosidase (0.517 ±
0.17 mg/ml), α-amylase (1.138 ± 0.42 mg/ml), and pancreatic lipase (0.809 ± 0.18 mg/ml) dose-
dependently. Compared to the disease control mice, treated experimental mice had near normal
blood glucose, HbA1c, and dyslipidaemia levels for 60 days. Weight increase and fatty tissue
accumulation were similarly reduced by A. hookerii. ADME filtration and metabolite profiling
revealed 28 compounds from flavonoids and phenolic acids. Network pharmacology analysis
confirmed that gene targets in insulin resistance, lipid and atherosclerosis, PI3K-Akt signalling,
insulin signalling, Type II diabetes, and metabolic pathways prevented non-insulin-dependent
diabetes and dyslipidaemia. A. hookerii has similar pharmacological potential to the reference
medication for non-insulin-dependent diabetes and dyslipidaemia. This research will guide the
development of nutraceuticals from food plants to prevent diabetes and metabolic disorders.

Keywords: Allium hookerii Thwaites; Metabolomics; Non-insulin dependent diabetes mellitus;
Dyslipidemia; Network Pharmacology