Page 195 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PP-13

Investigation of the anticancer property of an aqueous crude extract from

native Taiwan fern plant AC118-RLS

1
,1
1
Jie-Yu Liu, Cheng-Yun Li, Yi-Ching Juan, Min Liu*
1

1 Department of Life Science, Chinese Culture University, Taipei 113, Taiwan
* E-mail: lm@ulive.pccu.edu.tw

Abstract
Cancer accounts for 8.2 million deaths annually. Although chemotherapy is considered to
be the most effective treatment, undesirable side effects are unavoidable. For this reason,
complementary and alternative medicine becomes an additional choice alone with conventional
therapy. The purpose of this study is to investigate the antitumor bioactivity of aqueous crude
extracts collected from Taiwanese native fern plant AC118-RLS against human lung
adenocarcinomic cancer. Our previous studies revealed that addition of AC118-RLS
significantly reduced viability of human A549 adenocarcinoma lung cells in vitro in a dose
dependent manner. The treatment also increased the production of intracellular reactive
oxygen species and induction of apoptosis. In this study, we evaluated the antitumor property
of AC118-RLS in vivo using xenotransplantation animal model. C57BL6 mice were
inoculated with Lewis's LL/2 lung cancer cells and were divided into four groups and orally fed
with AC118-RLS for 28 days: control (0 mg/day), low-dose (4 mg/day), mid-dose (12 mg/day)
and high-dose (36 mg/day). Our results showed that administrator of AC118-RLS reduced
average tumor weight. Body weight, vital organ weight and histology, as well as hemoglobin
content, of all experimental groups were within normal physiological ranges, compared that to
of control group. In our future studies, we will test whether AC118-RLS treatment
synergistically enhanced the cytotoxicity of the first-line anticancer drugs against lung cancers.
This work documents the antitumor bioactivity of aqueous extract AC118-RLS from Taiwan
native fern against lung cancer and provides insights into the potential pharmacological
application of AC118-RLS as antitumor agent against lung cancer.

Keywords: Taiwan fern; Aqueous crude extract; Lung adenocarcinoma; Complementary and
alternative medicine