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PP-14

Formulation and evaluation of irritating and anti-inflammatory properties

of hydrogel containing Bryophyllum pinnatum (Lam.) Oken leaf extract

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Minh-Ngoc Thi Le, Quyen Hoang Do, Quyen T. Truong, Phuong Thi Diem Thach, Quy
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Van V o , Thi Thi Diem Nguyen, Anh Minh Tuan Nguyen, Duyen Thi My Huynh* ,1

1 Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, Can Tho City 900000,
Vietnam
* Email: htmduyen@ctump.edu.vn

Abstract
In Vietnamese traditional medicine, Bryophyllum pinnatum (Lam.) Oken (Crassulaceae) or
Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) is widely used for injury, infection, and
inflammation treatment. The current study was conducted to formulate a topical anti-
inflammatory gel based on Bryophyllum pinnatum (Lam.) Oken leaf extract and to assess the
preparation’s anti-inflammatory properties. Different excipients for gel preparation, including
carbopol 940, HPMC K4M, glycerin, and propylene glycol, were used and assessed in terms of
their spreadability, viscosity, and uniformity. The quality of all tested gel formulations was
evaluated with a special focus on their irritation activity using the HET-CAM method and their
anti-inflammatory action using the protein denaturation test. Consequently, the finalized gel
formulation comprised 0.8% leaf extract; 1% carbopol 940; 2.5% glycerin; 2.5% propylene
glycol; and excipients q.s. An IC50 value of 90.63±3.35 μg/mL in the protein denaturation test
and the HET-CAM method indicated that the obtained preparation has efficient anti-
inflammatory activity without in vitro irritation.

Keywords: Bryophyllum pinnatum (Lam.) Oken; Gel; Anti-inflammatory; HET-CAM