Page 280 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

CM-13

Comparative analysis of Jing Guan Fang and Jing Guan Liang Fang:

Enhanced modulation of viral entry and anti-inflammatory pathways

1,2
#,1
1
Hsin Yeh, Zhi-Hu Lin, Hung-Chih Lo, Chung-Hua Hsu, Tung-Yi Lin *,1,2
1

1 Institute of Traditional Medicine, National Yang Ming Chiao Tung University, Taipei,
112304, Taiwan
2 School of Chinese Medicine, National Yang Ming Chiao Tung University, Taipei, 112304,
Taiwan
* E-mail: biotungyi@gmail.com/tylin99@nycu.edu.tw

Abstract
Jing Guan Fang (JGF) is a classical herbal prescription traditionally applied to strengthen
immunity and support respiratory health, particularly in response to viral infections. To
optimize its therapeutic benefits, a modified formula, Jing Guan Liang Fang (JGLF), was
developed by adjusting the herbal composition and incorporating additional
immunomodulatory constituents. This study aimed to compare their chemical profiles and
biological activities, with emphasis on antiviral and anti-inflammatory effects. Cytotoxicity
assessment by MTT assay confirmed good safety across the tested ranges. In WI-38 cells, both
formulas transiently suppressed ACE2 expression within 3 h, while TMPRSS2 reduction
persisted for 24 h, suggesting potential regulation of viral entry pathways. In LPS-stimulated
RAW264.7 macrophages, JGF and JGFL both inhibited IL-6 and nitric oxide (NO) production.
JGFL demonstrated markedly stronger activity, with EC50 values of 169.36 μg/mL for IL-6
inhibition and 17.95 μg/mL for NO suppression, compared to 167.01 μg/mL and 87.07 μg/mL
for JGF, respectively. Western blot analysis further showed that JGFL downregulated iNOS and
COX-2 expression and reduced phosphorylation of STAT3 and JAK2, indicating modulation of
inflammatory signaling cascades. HPLC profiling revealed compositional differences: JGF
contained baicalin, wogonoside, and oroxyloside, whereas JGFL was enriched in baicalin,
wogonoside, and baicalein. Collectively, these results highlight that JGFL not only transiently
modulates viral entry receptors but also exhibits enhanced anti-inflammatory effects,
supporting its potential as a promising adjunctive therapy for respiratory infectious diseases.

Keywords: Jing Guan Fang; Jing Guan Liang Fang; Herbal formulation; Antiviral activity;
Phytochemical analysis