Page 282 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

CM-15

The identification and extraction of plant-derived exosome-like nanovesicles

from Dendrobium fimbriatum Hook and their anti-inflammatory applications

3,4
5
1,2
Aimee Hong, Chuan-Teng Liu, Michael Heinrich, Ying-Chyi Song, Hung-Rong
2
Yen*
,1,2

1 School of Chinese Medicine, College of Chinese Medicine, China Medical University,
Taichung 404328, Taiwan
2 Research Center for Traditional Chinese Medicine, Department of Medical Research, and
Department of Chinese Medicine, China Medical University Hospital, Taichung 404327,
Taiwan
3 School of Pharmacy, University College London, London WC1N 1AX, United Kingdom
4 Department of Pharmaceutical Sciences and Chinese Medicine Resources, and Chinese
Medicine Research Center, China Medical University, Taichung 404328, Taiwan
5 Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical
University, Taichung 404328, Taiwan
* E-mail: hungrongyen@mail.cmu.edu.tw

Abstract
Dendrobium fimbriatum Hook (DF) is a medicinal plant traditionally used in Chinese
medicine. Recent studies have revealed Dendrobium species possessing antioxidant properties,
largely attributed to their high polysaccharide content. Plant-derived exosome-like nanovesicles
(PDENs) present as promising therapeutic agents due to their natural origin, low cost, and
capacity for intercellular communication. This project aimed to explore the immunomodulatory
potential of DF-derived PDENs (DF-PDENs) as a foundation for developing novel therapies
for inflammatory skin disorders. DF stems from 1-, 2-, and 3-year old plants were harvested
from Sun Ten Pharmaceutical Company’s farm in Nantou, Taiwan. DF-PDENs were prepared
using ultracentrifugation and sucrose-density gradient ultracentrifugation. Yield and purity of
PDENs extracted from each method were evaluated by nanoparticle tracking analysis. TNF-α-
and IL-17-induced keratinocyte models were used to assess the anti-inflammatory effects and
cytotoxicity, with IL-6 and IL-8 levels measured to elucidate in vitro effects and mechanisms.
Ultracentrifugation yielded the highest purity PELNs, as confirmed by nanoparticle tracking
analysis. DF-PDENs significantly reduced inflammatory responses in TNF-α- and IL-17-
stimulated keratinocytes, accompanied by decreased secretion of IL-6 and IL-8. DF-PDENs
showed anti-inflammatory activity in keratinocytes, supporting their potential as treatments for
inflammatory skin diseases.

Keywords: Plant-derived exosome-like nanovesicle; Dendrobium fimbriatum Hook;
Keratinocyte