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CM-18

The relationship between chemical profiles and bioactivities of

Helminthostachys zeylanica from different Regions.

#,1
3
2
Mao Xuan Honga, Kuan-Ying Lai, Tsong-Long Hwong, Y u -Liang Yang, Y u -Chung
1
4
Chiang, Chih-Chuang Liaw* ,1,5

1 Department of Marine Biotechnology and Resources, National Sun Yat-sen University,
Kaohsiung 80424, Taiwan
2 Research Center for Chinese Herbal Medicine, Graduate Institute of Health Industry
Technology, College of Human Ecology, Chang Gung University of Science and Technology,
Taoyuan, 33303, Taiwan.
3 Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan
4 Department of Biological Science, National Sun Yat-sen University Academia Sinica,
Kaohsiung 80424, Taiwan.
5 Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University
Academia Sinica, Kaohsiung 80424, Taiwan.
* E-mail: ccliaw@mail.nsysu.edu.tw

Abstract
The rhizomes of Helminthostachys zeylanica (L.) Hook, traditionally known as Daodi-Ugon
in Chinese medicine, have long been used for the treatment of inflammation and various hepatic
disorders. In our previous study, two commercial-available products, Hong Daodi-Ugon (HZR)
and Hei Daodi-Ugon (HZB), were shown to differ in their flavonoid glycoside content,
consistent with in vitro results supporting the claim that HZR exhibits superior efficacy
compared to HZB. However, aside from differences in color, no clear criteria have been
established to distinguish these materia. To investigate the origns of these products, we tried to
collected H. zeylanica samples from multuple regions, including China (imported, HZC),
Indonesia (imported, HZI), Sri Lanka (collected by ourselves, HZSL), Thailand (collected by
ourselves, HZT), and Taiwan (collected by ourselves, HZFS and HZM). All samples were
sequentially extracted using a gradient-solvent system (n-hexane, DCM, MeOH, and 50%
MeOH). The MeOH (M) and 50% MeOH (50M) extracts were further analyzed using an
optimized HPLC system. The HPLC profiles indicated that HZC and HZI were chemically
similar to the commercial HZB, characterized by higher levels of flavonoid glycosides with 4-
5 sugar units. In contrast, HZT, HZFS, and HZM resembled the commercial HZR, enriched in
flavonoid glycosides with 2-3 sugar units. Anti-inflammatory assays demonstrated that HZFS-
50M, HZFS-M, HZM-50M, and HZM-M significantly inhibited superoxide anion generation
and elastase release in fMLF/CB-induced human neutrophils at 10 μg/mL. In addition, the
retina-related activities of these extracts were also evaluated. These findings provide valuable
point on material selection and further therapeutic applications of H. zeylanica in traditional
Chinese Medicine.

Keywords: Helminthostachys zeylanica; Flavonoid glycosides; Ugonins; anti-inflammatory
activity