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PC-13

Chemical constituents from Juniperus formosana needle extracts and their

anti-inflammatory activity

1
2,3
1
Hong Nhung Phan, Hao-Ze Li, Tsong-Long Hwang, Mei-Lin Yang, Ping-Chung
1
Kuo*
,1

1 School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 70101,
Taiwan
2 Research Center for Chinese Herbal Medicine and Graduate Institute of Healthy Industry
Technology, College of Human Ecology, Chang Gung University of Science and
Technology, Taoyuan, Taiwan
3 Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, Taiwan
* E-mail: z10502016@ncku.edu.tw

Abstract
Juniperus formosana is an endemic plant of the Juniperus genus, and is found in sand land,
rock gaps, and sunny hillsides. In traditional medicine, the stems and roots of J. formosana
were used for treating measles, clearing heat, and stopping bleeding. Furthermore, various
bioactive compounds, including flavonoids, lignans, diterpenes, sesquiterpenes, and
phenylpropanoids, were noted for J. formosana. Moreover, many biological activities,
including antibacterial, antiviral, anti-inflammatory, anticancer, and anticytotoxic properties,
have been verified in this plant. In the preliminary examination, the J. formosana extracts were
examined for their inhibition activities of superoxide anion generation and elastase release in
fMLF/CB-induced human neutrophils, showing an outstanding result with the ethyl acetate
extract. Therefore, this study aims to explore the bioactive components of the ethyl acetate
extract of J. formosana and evaluate the anti-inflammatory bioactivity of these constituents on
a cellular neutrophil. As a result, seventy compounds were found in this study, and the
identification of structures was carried out by comparing the spectroscopic data with the
published information. Among them, three undescribed compounds were characterized based
on the extensive spectrometric analyses. All these isolated compounds will be subjected to the
evaluation of their anti-inflammatory potential by the human neutrophil model.

Keywords: Cupressaceae; Anti-inflammatory; Human neutrophil; Lignan; Flavonoid