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PC-30

Anti-neuroinflammatory polyketides from the medicinal herb-derived

fungal strain Didymocyrtis brachylaenae Km1530

1
1
1
Chia-Hao Chang, Yun-Rong Zhang, Shu-Jung Huang, George Hsiao, Wei-Chiung
2,3
1
Chi, Andrea Gu, Y u -Wei Lai, Tzong-Huei Lee* ,1,6
4
5

1 Institute of Fisheries Science, College of Life Science, National Taiwan University, Taipei
106319, Taiwan
2 Department of Pharmacology, School of Medicine, Taipei Medical University, Taipei
110301, Taiwan
3 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University,
Taipei 110301, Taiwan
4 Department of Food Science, National Quemoy University, Kinmen 892009, Taiwan
5 Center of General Education, National Taipei University, New Taipei 273303, Taiwan
6 Department of Life Science, College of Life Science, National Taiwan University, Taipei
106319, Taiwan
* E-mail: thlee1@ntu.edu.tw

Abstract
Chromatographic separation on the liquid-state fermented products of the littoral medicinal
herb Atriplex maximowicziana Makino-derived fungal strain Didymocyrtis brachylaenae
Km1530, resulted in the isolation of compounds 1–13. Their structures were determined by
spectroscopic analysis as five previously unreported C13 polyketides, namely brachylactones
A–D (1–4) and brachic acid (5), along with 3-methylorsellinic acid (6), 2-(2-
hydroxyacetyl)furan (7), (+)-pestalotiolactone A (8), cyclo[L-(4-hydroxyprolinyl)-L-leucine]
(9), phenylacetic acid (10), N-acetyltyramine (11), 4-hydroxyphenylacetic acid (12), and methyl
2-(4-hydroxyphenyl)acetate (13). Among these, brachylactones A (1) and B (2) exhibited
moderate nitric oxide production inhibitory activity in lipopolysaccharide-induced murine BV-
2 microglial cells at a concentration of 20 μM without any cytotoxicity.

Keywords: Anti‑neuroinflammation; Brachylactone; Didymocyrtis brachylaenae; Nitric oxide;
Polyketide