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PC-31

Isolation and structure elucidation of secondary metabolites from the

seagrass-associated fungus Aspergillus niger.

1
,1
Muhammad Ali, Yuan Bin Cheng*

1 Department of Marine Biotechnology and Resources, National Sun Yat-sen University,
Kaohsiung 804201, Taiwan
* E-mail: jmb@mail.nsysu.edu.tw

Abstract
Aspergillus niger is one of the most common and important fungal species, and is ubiquitous
in various environments. A. niger isolates possess a large number of biosynthetic gene clusters
(BGCs) and produce a wide range of secondary metabolites, with applications spanning
agriculture, food, and the pharmaceutical industry. The current study aimed to isolate secondary
metabolites of A. niger with diverse structural features. We identified eighteen secondary
metabolites belonging to eight different skeletal cores. They are diphenyl ether derivatives (1-
5), benzophenones (6 and 7), spirocyclic polyketide (8), hetero-spirocyclic γ-lactam derivatives
(9 and 8), coumarin derivative (11), anthraquinone derivatives (12 and 13), diketoperazine
alkaloid derivatives (14 and 15), helvolic acid and its derivatives (16-18). Notably, the
compounds 1 and 3 were identified as new metabolites, and their structures were determined
by extensive spectroscopic analysis using NMR (1D and 2D), HREIMS, UV, and IR
spectroscopy. Additionally, sixteen known compounds were characterized based on
spectroscopic data and compared with the literature.

Keywords: Aspergillus niger; Diphenyl ether; Diketopiperazine alkaloids; Helvolic acid.