Page 153 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PC-35

Isolation and structural elucidation of natural products from the Formosan

octocoral Lobophytum durum

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Chia-Ling Hung, #,1,2,3 Hao-Yu Liao, Fang-Yu Wu, Y u -Rou Pan, Hsiang-Ting Lo, Jun-
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Wen Chen, Y a -Lun Cai, Kun-Miao Lai, Xin-Yun Xie, Jia-Ling Hong, Hsu-Ming
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Chung*

1 Department of Marine Biotechnology and Resources, National Sun Yat-sen University,
Kaohsiung 804201, Taiwan
2 Department of Chemistry, Republic of China Military Academy, Kaohsiung 83059, Taiwan
3 Department of Applied Chemistry, National Pingtung University, Pingtung 900391, Taiwan
* E-mail: shiuanmin@mail.nptu.edu.tw

Abstract
Eleven compounds (1–11) were isolated from the Formosan octocoral Lobophytum durum
collected off Guishan Island, Yilan, Taiwan. A new sterol, 3β-hydroxy-25-methylene-5-
cholesten-7-one (1), was identified along with ten known metabolites: (22R,23R,24R)-5α,8α-
epidioxy-22,23-methylene-24-methylcholest-6-en-3β-ol (2), 7-oxogorgosterol (3), 24R-

methylcholestane-3β,5α,6β,25-tetrol 25-monoacetate (4), 20-acetylsinularolide C (5), 20-
acetylsinularolide B (6), sinularolide B (7), sinularolide C (8), lobolide (9), lobophytolide D
(10), and 1-O-octadecyl glycerin ether (11). Structural assignments were confirmed by
spectroscopic analyses and literature comparison. Compounds 2–4 and 11 represent the first
isolation from this species, and the absolute configuration of compound 4 was determined by

X-ray crystallography. All isolates were tested for anti-inflammatory activity, assessing their
inhibitory effects on ROS and elastase release in human neutrophils.

Keywords: Lobophytum durum; ROS; Elastase; X-ray; IC50