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PC-23

Phytochemical biomass production and isolation of dehydroeburicoic acid,

an ALDH2 activator, from Antrodia salmonea in the ABF Germplasm Bank

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1
Hsien-Hung Chang, #,1 Mohamed El-Shazly, Chih-Li Chen, Y u -Ming Hsu, Ying-Chi
2
1,4
,1
1
Du, Y u -Hsuan Lin, Shih-Tsun Liang, Tun-Tschu Chang, Chuen-Hsu Fu*
1
1

1 Taiwan Forestry Research Institute, Ministry of Agriculture, Taipei 100051, Taiwan
2 Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Organization
of African Unity Street, Abassia, Cairo 11566, Egypt
3 Research Center for Precision Environmental Medicine, Kaohsiung Medical University,
Kaohsiung City 807378, Taiwan
4 Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944401,
Taiwan
* E-mail: fch@tfri.gov.tw

Abstract
Aldehyde dehydrogenase 2 (ALDH2) is a key mitochondrial enzyme involved in oxidizing
aldehydes, notably those derived from lipid peroxidation under oxidative stress. Recent studies
highlight ALDH2’s critical role in metabolizing endogenous and exogenous aldehydes, offering
therapeutic potential for managing cardiovascular diseases, obesity, fatty liver, glucose
dysregulation, and aging-related disorders through improved mitochondrial function.
Dehydroeburicoic acid (DEA), an ALDH2 activator derived from the medicinal fungus
Antrodia salmonea, enhances ALDH2 activity, promoting the clearance of reactive aldehydes
like 4-HNE in the liver. This reduces oxidative stress and inflammation, stimulates fatty acid β-
oxidation, and suppresses lipogenesis. The "Phytochemical Biomass Production Study"
explores how environmental factors, such as nutrient availability, light, and cultivation
techniques, affect phytochemical yield in plants and fungi, aiming to optimize biomass for
pharmaceutical and nutraceutical applications. Established in 2021, the Applied Biomedical
Fungal Germplasm Bank (ABF Germplasm Bank) focuses on evaluating biomass and
pharmacochemical properties of biomedical fungi. In this study, we developed an efficient
protocol for isolating DEA from liquid-cultured Antrodia salmonea using reflux extraction and
liquid-liquid partitioning. The DEA-rich fraction yield was quantified, and its phytochemical
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profile was characterized via RP-HPLC-UV and H-NMR analyses.

Keywords: Aldehyde dehydrogenase 2 (ALDH2); Phytochemical biomass production; fungal
germplasm bank; Dehydroeburicoic acid (DEA); Antrodia salmonea