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PC-24

Monosaccharide composition, structural characterization, and immuno-

modulatory effect of polysaccharide from Sarcodia suiae

1
2
2
Wei-Cheng Yuan, Yi-Cheng Pan, Yeong-Jiunn Jang, Chia-Lin Lee,* ,2,3 Yang-Chang
Wu* ,1,2

1 Master Program of Pharmaceutical Manufacture, College of Pharmacy, China Medical
University, Taichung 40402, Taiwan

2 Chinese Medicine Research and Development Center, China Medical University Hospital,
Taichung 40447, Taiwan
3 Department of Cosmeceutics, China Medical University, Taichung 40604, Taiwan
* E-mail: chlilee@mail.cmu.edu.tw (C.-L. Lee); yachwu@mail.cmu.edu.tw (Y.-C. Wu).

Abstract
Sarcodia suiae, a red macroalga of the genus Sarcodia within the phylum Rhodophyceae, is
predominantly distributed throughout the Indian Ocean and the Western Pacific Ocean. Valued
for its rich micronutrient content, S. suiae is widely consumed both as a food additive. Previous
studies have demonstrated that S. suiae extracts exhibit diverse bioactivities, including

immunomodulatory, antitumor, and anti-inflammatory effects. However, comprehensive
research on the detailed structural features and comparative analysis of S. suiae-derived
polysaccharides remains limited. SSP-1, a purified polysaccharide isolated from SSP, was
obtained via HPLC using a size-exclusion column. Based on the linear regression equation

derived from dextran molecular weight standards, the molecular weight of SSP-1 was estimated
to be 2798.98 kDa. The purified polysaccharide exhibited a carbohydrate content of 84.20%,
negligible protein content, and 34.3% of sulfate content. Monosaccharide composition analysis
classified SSP-1 as a heteropolysaccharide comprising galacturonic acid, glucose, galactose,

and xylose. Among these, galactose was the main sugar, accounting for 84.69% of the total
monosaccharide content, followed by xylose (7.54%), galacturonic acid (6.63%), and glucose
(1.14%). The IR spectrum demonstrated adsorbances with functional groups with sulfate. These
results suggest that SPP-1 was likely constitutes with galactose and contained sulfate functional

groups, which may belong to the calss of carrageenan. The study presents the first report to
investigate the molecular weight, monosaccharide composition and potential bioactivities of a
polysaccharide derived from S. suiae. Further analysis will focus on elucidating the detailed
glycosidic linkages, structural characterization, and immunomodulatory effects of SSP-1, with

the aim of evaluating its potential applicability in biomedical and functional health product
development.

Keywords: Sarcodia suiae; Red macroalga; Polysaccharide; Galactose; Immunomodulatory