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PC-21

Isolation and identification of marine natural products from Aspergillus

ochraceopetaliformis

,1
#,1
Yi-Lin Tsai, Yuan-Bin Cheng*

1 Department of Marine Biotechnology and Resources, National Sun Yat-sen University,
Kaohsiung 80424, Taiwan
* E-mail: jmb@mail.nsysu.edu.tw

Abstract
In this study, a strain of Aspergillus ochraceopetaliformis was isolated from the sea anemone
Palythoa mutuki, representing a promising marine-derived microorganism. Preliminary
cytotoxicity assays revealed that this strain can produce metabolites with antitumor activity.
Genome analysis combined with molecular networking was further employed to explore the
biosynthetic potential of this strain and to predict putative bioactive secondary metabolites.
Large-scale fermentation, followed by successive extraction, chromatographic separation, and
purification, yielded a series of secondary metabolites, including fifteen new compounds (1–
15) and twelve known compounds (16–27). These metabolites belong to asteltoxin derivatives,
crotonic acid derivatives, and terpenoids. The structures of the isolated metabolites were
elucidated using a combination of nuclear magnetic resonance (NMR), high-resolution
electrospray ionization mass spectrometry (HR-ESI-MS), X-ray diffraction analysis, and
comparisons of ECD data, leading to the identification of several compounds. These findings
highlight the potential of A. ochraceopetaliformis as a valuable source of bioactive natural
products and provide a foundation for further investigation into their pharmacological
applications.

Keywords: Aspergillus ochraceopetaliformis; Palythoa mutuki; Antitumor activity; genome
analysis; Molecular networking