Page 138 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PC-20

Purification, chemical characterization, and in vitro anti-cancer activity of a

fucose-rich sulfated polysaccharides from Ganoderma lucidum

2,3
2,3
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Yi-An Lin, Wei-Lun Qiu, Chi-Hsein Chao, Mei-Kuang Lu, 1,2,3 Tung-Yi Lin* ,1,2,3

1 Institute of Traditional Medicine, School of Medicine, National Yang Ming Chiao Tung
University, Taipei, Taiwan
2 School of Chinese Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan
3 Traditional Chinese Medicine Glycomics Research Center, National Yang Ming Chiao Tung
University, Taipei, Taiwan
* E-mail: biotungyi@gmail.com

Abstract
Ganoderma lucidum polysaccharides (GLPs), especially their sulfated derivatives (GSPS),
have shown considerable therapeutic promise, particularly in cancer treatment. Previous studies
have demonstrated that GSPS can inhibit tumor progression by targeting key oncogenic
signaling pathways. In this study, GSPS was further purified from G. lucidum mycelia. Two
fractions, J101-F2 and F3, were isolated using gel filtration chromatography. Their structural
properties were analyzed via high-performance anion-exchange chromatography, acid
hydrolysis for sulfate content, and molecular weight determination. Functional assessments
included anticancer activity tests and synergy assays with clinical chemotherapeutic agents. F2
contained high levels of fucose (35.83%) and galactose (23.13%), while F3 was rich in glucose
(29.18%) and fucose (24.55%). Sulfate content and molecular weight were also higher in F2
(4.58 mmol/g, 485.76 kDa) than in F3 (3.77 mmol/g, 231.78 kDa). Both fractions suppressed
cancer cell proliferation and induced apoptosis in lung and colorectal cancer cells. These effects
were enhanced when combined with chemotherapeutic agents, resulting in sub-G1 cell cycle
arrest and activation of intrinsic apoptotic signaling. Structural differences between F2 and F3,
especially in sulfate content and size, may underlie their distinct bioactivities. By modulating
oncogenic signaling and inducing apoptosis, they act through multiple mechanisms. Their
synergy with chemotherapy supports their potential as adjuncts in integrative cancer treatment.

Keywords: Ganoderma lucidum; Sulfated Polysaccharides; Apoptosis