Page 154 - 2025中醫藥與天然藥物聯合學術研討會-中醫藥與天然藥物的挑戰Ｘ機遇與未來大會手冊

PC-36

Evaluation of secondary metabolites of Streptomyces spp. in controlling

Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense

*,3
1,2
Y a -Ling Hou, #,1,2,3 Bo-Wei Wang, Hui-Chun Wang, Y u -Liang Yang* ,1,2,3

1 Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115201, Taiwan.
2 Biotechnology Center in Southern Taiwan, Academia Sinica, Tainan 711010, Taiwan
3 Graduate Institute of Natural Product, Kaohsiung Medical University, Kaohsiung 807378,
Taiwan
* E-mail: wanghc@kmu.edu.tw, ylyang@gate.sinica.edu.tw

Abstract
Fusarium wilt of banana (FWB), caused by the soil-borne fungus Fusarium oxysporum f. sp.
cubense Tropical Race 4 (Foc TR4), is one of the most destructive diseases threatening global
banana production. Without effective and sustainable disease-management strategies,
biological control has emerged as an important alternative. Actinomycetes are considered
valuable biocontrol resources due to their capacity to produce diverse secondary metabolites.
In this study, four Streptomyces spp. (D1, D3, D4, and D4c) were isolated from the banana
rhizosphere and exhibited strong inhibitory activity against both spore germination and
mycelial growth of Foc TR4. By integrating genomic and metabolomic approaches, including
whole-genome sequencing followed by antiSMASH analysis, and LC-MS/MS followed by
Feature-Based Molecular Networking (FBMN), we identified diverse biosynthetic gene
clusters and secondary metabolites with antifungal potential. To further the evaluate
applicability and biosafety, greenhouse trials were conducted in potted banana plants, and
cytotoxicity of secondary metabolites was evaluated in SF-9 insect cells using CCK-8 assays.
Secondary metabolites derived from Streptomyces sp. D4 resulted in significant mitigation of
Fusarium wilt symptoms without detectable cytotoxicity. Collectively, these findings highlight
the importance of Streptomyces sp. D4 is a promising biocontrol agent for development into an
environmentally friendly application.

Keywords: Streptomyces; Fusarium wilt of banana; Antifungal secondary metabolites;
Biocontrol